Medical Office Assistant · Clinical Office Practice and Patient Safety

Disinfection and Sterilization

On this page 3 sections
  1. Quick check
  2. Study tools
  3. Sources & references

Quick check

5 questions here, of 90 in this lesson’s practice set. Answers stay hidden until you check.

Question 1 of 5foundational

Which statement best describes sterilization?

Choose an answer, then check it.
Question 2 of 5foundational

What is high-level disinfection?

Choose an answer, then check it.
Question 3 of 5foundational

Which microorganism is the key factor that distinguishes sterilization from high-level disinfection?

Choose an answer, then check it.
Question 4 of 5intermediate

Place the following in order from the lowest to the highest level of microbial destruction: low-level disinfection, cleaning, high-level disinfection, sterilization.

Choose an answer, then check it.
Question 5 of 5intermediate

An item will contact the sterile tissues inside a patient's body during a procedure. What level of processing is required?

Choose an answer, then check it.
Practice all 90

Keep learning

Ready to build on this? Continue to the next lesson.

Practice this lesson
Study tools & related lessonsYou’ll learn to · Related

You’ll learn to

  • Distinguish high-level disinfection from sterilization, including what each process does and does not accomplish.
  • Describe the methods of sterilization used in ambulatory settings, with emphasis on steam (autoclave) principles and limitations.
  • Apply the Spaulding classification to determine whether a given item requires cleaning, disinfection, high-level disinfection, or sterilization.
  • Explain how sterilization is monitored using mechanical, chemical, and biological indicators, and the appropriate response to failures.
  • Recognize the medical office assistant's role in reprocessing and monitoring, including following manufacturer instructions, facility policy, and escalating problems.

Sources & references

  1. Centers for Disease Control and Prevention — Infection Control
  2. Occupational Safety and Health Administration — Bloodborne Pathogens
  3. U.S. National Library of Medicine — MedlinePlus

This lesson was adapted from the open educational references above; their licenses and attributions are preserved. See Copyright & Licensing.

Educational content only. It is not medical, legal or professional advice. Found an error? Tell us.